Coding
FadA

Part:BBa_K861400

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-08-16)

For E.coli to degrade fatty acid, at least four enzymes – FadA, FadB, FadD, FadE and a transporter—FadL are needed. Long chain fatty acids(FAs) are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthetase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The following cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
    Illegal BglII site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 574
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
//direction/forward
Parameters
n/aFadA protein coding sequence for fatty acid degradation